Phage Formation in Staphylococcus Muscae Cultures
نویسنده
چکیده
1. The release of S. muscae phage in veal infusion medium is correlated with lysis of the host. 2. The release of the bacterial virus in Fildes' synthetic medium occurs in a step-wise manner before observable lysis of the cells occurs. This result has been confirmed by both turbidimetric readings and direct microscopic examination of the infected cells.
منابع مشابه
PHAGE FORMATION IN STAPHYLOCOCCUS MUSCAE CULTURES X. THE RELATIONSHIP BETWEEN VIRUS SYNTHESIS, THE RELEASE 0]? BACTERIAL RIBONUCLEIC ACID, VIRUS LIBERATION, AND CELLULAR LYSlS BY WINSTON H. PRICE*
Previous experiments have indicated that infected cells of strain I of S. muscae may release phage under certain conditions without lysing (1). Lwoff and Gutman have recently suggested that the phage is actually liberated while the sample is being diluted for the phage assay, with the cells undergoing lysis at this time (2). I t therefore seemed desirable to repeat these experiments in such a w...
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In the preceding paper (1) it was found that some strains of S. muscae required the addition of a substance found in certain acid-hydrolyzed proteins (AHPF) to liberate phage in Fildes' synthetic medium when singly infected with virus. Another strain required aspartic acid to liberate phage in Fildes' synthetic medium when singly infected. Experiments reported in this paper show that such strai...
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E. coli B and Staphylococcus muscae rendered non-viable by aqueous solutions of mustard gas at pH 7.5 to 8 can still produce phage.
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Previous studies from this laboratory have shown that resting cells of S. muscae have approximately the same amount of ribonucleic acid (RNA) and desoxyribonucleic acid (DNA). During the early growth phases, when there is little or no cellular multiplication, both the RNA and DNA content of the cells begin to increase. When the RNA content per cell has increased to 2 to 21⁄2 times over the DNA ...
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The action of mustard gas on six animal, one plant, and two bacterial viruses; also on bacteria, yeast, and the pneumococcus-transforming principle has been studied. The viruses include Newcastle's disease of chickens, equine encephalomyelitis (Eastern strain), feline pneumonitis (Baker), rabbit papilloma (Shope), fixed rabies, rabbit myxoma, tobacco mosaic, T(2)r(+) phage of E. coli B, and a S...
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عنوان ژورنال:
- The Journal of General Physiology
دوره 33 شماره
صفحات -
تاریخ انتشار 1948